ENGINEERING VERO CELLS TO SECRETE HUMAN INSULIN
نویسندگان
چکیده
منابع مشابه
Characterisation of BHK-21 cells engineered to secrete human insulin.
Autoimmune destruction of beta cells in the pancreas leads to type I, or insulin dependent diabetes mellitus (IDDM), through the loss of endogenous insulin production capacity. This paper describes an attempt to generate 'artificial'beta cells using the fibroblast cell line BHK21. Stable transfectants expressing the human preproinsulin (PPI) gene were isolated and characterised. The resulting c...
متن کاملHuman bone marrow mesenchymal stem cells transfected with human insulin genes can secrete insulin stably.
Beta-cell replacement therapy by pancreatic islet transplantation has become a promising treatment for type 1 diabetes. However, the limited supply of human islet tissue prevents this therapy from being widely used to treat patients with type 1 diabetes. In order to obtain insulin-secreting cells, retrovirus vector pLNCX was used to transfer the human insulin gene into human bone marrow mesench...
متن کاملIn-vitro Differentiation of Human Umbilical Cord Wharton’s Jelly Mesenchymal Stem Cells to Insulin-Producing Cells
Background & Objective: Diabetes is a major chronic metabolic disease in the world. Islet transplantation is a way to treat diabetes. Unfortunately, this method is restricted due to graft rejection and lack of donor islets. Mesenchymal Stem Cells (MSCS) have the ability to differentiate into Insulin-Producing Cells (IPCs). In this study, Human Umbilical Mesenchymal Stem Cells (HUMSCS) were in...
متن کاملHuman Umbilical Vein Endothelial Cells Secrete Transcobalamin
Transcobalamin II (TC Ill is essential for cellular uptake of cobalamin. However, t h e origin of this t ransport protein is controversial and many organ sources have been suggested. We studied human umbilical vein endothelial cells cultured in vitro. The cells contained T C II (2.3 pmol/108 cells) and released progressively increasing amounts of t h e protein into t h e surrounding medium duri...
متن کاملPancreatic -Cells Secrete Insulin in Fast- and Slow-Release Forms
Insulin vesicles contain a chemically rich mixture of cargo that includes ions, small molecules, and proteins. At present, it is unclear if all components of this cargo escape from the vesicle at the same rate or to the same extent during exocytosis. Here, we demonstrate through real-time imaging that individual rat and human pancreatic -cells secrete insulin in heterogeneous forms that dispers...
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ژورنال
عنوان ژورنال: In Vitro Cellular & Developmental Biology - Animal
سال: 2002
ISSN: 1543-706X
DOI: 10.1290/1071-2690(2002)038<0146:evctsh>2.0.co;2